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Samples were vortexed again, centrifuged (16,000 g , 10 min, 4 °C) and then all supernatant was removed to leave a protein pellet
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However, maternal prophylaxis with high-titer anti-D immunoglobulin G (RhoGAM), combined with aggressive fetal surveillance and intrauterine blood transfusions, has greatly reduced the incidence and severity of this disease
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