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In vitro BP incorporation was performed at 37 Β°C for 1 h, and then, the samples were diluted with sample loading buffer (60 mM Tris-Cl (pH 6.8), 10% glycerol, 2% SDS, 1% Ξ²-mercaptoethanol (ME), 0.01% bromophenol blue) and boiled at 100 Β°C for 10 min
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Furthermore, for the isoprenoid tail to be attached, 4-HB must be transported into the mitochondrial matrix, where the condensation with the tail occurs
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